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Single Cellome? Unit SU10

Single - cell targeting with direct delivery into nucleus or cytoplasm.
Minimal damage to cells.
Automated, high speed, and high success rate.


Detail

Brief Introduction

Single Cellome Unit SU10 enables the delivery of substances, such as genome editing tools, directly to the cytoplasm or nucleus of targeted single cells.
For use with an inverted optical microscope. The microscope is not included with the SU10.


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Product Characteristics

1、Single-cell targeting with direct delivery into nucleus or cytoplasm

  • Select cells for delivery while observing under a microscope and deliver substances to into nucleus or cytoplasm of targeted cells.

  • Easy Control of XY-position and automatic control of delivery Z-position in software.


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FITC-labelled dextran solution (molecular weight 70,000) was delivered into HeLa cells.


2、Minimal damage to cells

  • The nanopipette is a glass pipette with minimum tip outer diameter of several tens of nanometers, minimizing damage to cells.

  • Delivery with high cell viability enables live single-cell analysis.


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3、Automated, high speed, and high success rate


  • The SU10 uses automated cell surface detection, insertion, and delivery to the cell. The process takes approximately 10 seconds with a 90% success rate. (Experiment by Yokogawa)

  • Operations that were previously performed manually by experienced researchers are much easier with SU10


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Application Fields

The typical applications are including:

  • Delivery of genome editing tools directly into the nucleus.

  • Efficacy/toxicity evaluation of drug candidate molecules.

  • Delivery into cells that cannot be introduced by conventional methods.

  • Analysis of cell-cell communication starting from the injected cells.

 

Examples of cells that can be targeted:

  • Cell line (HeLa, HEK293, CHO, MDCK, HepG2, etc.)

  • Primary cell culture (Neuron, Hepatocyte, etc.)

  • Stem cell lines (iPS cell, ES cell, etc.)

  • Immune cell lines (T cell, Ba/F3 cell, etc.)

  • Plant cell with cell wall

 

Examples of materials that can be injected:

  • CRISPR-Cas9 (Cas9 RNP)

  • Protein (antibody, GFP, etc.)

  • Nucleic acid (single-stranded DNA, etc.)

  • Fluorescent reagent

 


Application

1、Delivery of genome editing tools directly into the nucleus.

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2、Efficacy/toxicity evaluation of drug candidate molecules.

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3、Delivery into cells that cannot be introduced by conventional methods.

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4、Analysis of cell-cell communication starting from the injected cells.

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Video

SU10 Introduction Video

SU10 Introduction Video

Case

Message

森西賽智科技是集自主研發(fā)、海外先進(jìn)技術(shù)國內(nèi)整合轉(zhuǎn)化和引進(jìn)海外前沿產(chǎn)品為一體的生命科學(xué) 儀器專業(yè)服務(wù)平臺(tái)。